Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 25G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 25G
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.5) - 100ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sigma Aldrich Fine Chemicals Biosciences ACES >=99.0% (titration) | 7365-82-4 | MFCD00008030 | 25G
ACES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 182.2 | 7365-82-4 | MFCD00008030 | 25G
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 1KG
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 1KG
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 100g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 100g
Research Products International Corp Bicine Ultra-Pure, 100 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Alkali Scientific CellPro™ HEPES Buffer, 0.5M, pH7.0, 125 ml bottle
CellPro™ HEPES Buffer, 0.5M, pH7.0, 125 ml bottle
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 1KG
MOPS sodium salt, 71119-22-7, MFCD00064350, 1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 25g
HEPES | Purity: 99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 25g
Bioworld PIPES BUFFER 1M PH 6.0 100ML
liquid, 1.0m, ph 6Synonym(s): 1,4-Piperazinediethanesulfonic acidMolecular Formula: C8H18N2O6S2CAS Number: 5625-37-6Molecular Weight: 302.37
Medchemexpress LLC MES monohydrate (Standard) | 145224-94-8 | MFCD00149409 | 100.0% | 25 MG
MES monohydrate (Standard) is an analytical standard and an amphoteric ion buffer used in research and analytical applications. This compound, also known as 2-Morpholinoethanesulphonic acid, is a Good's buffer with a capacity ranging from pH 5.5-7.0.
- Analytical standard for research and analytical applications
- Functions as an amphoteric ion buffer
- Offers a buffer capacity from pH 5.5-7.0
- Widely used in biochemistry and molecular biology
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Utilized in qualitative, quantitative, and methodological research experiments
- Compatible with techniques such as HPLC, GC, and MS